Huismans MA, Smabers LP, Brunner SR, van Hoeck A, Kaa DV, Franken IA, Wensink E, Koster J, Volckmann R, Kranenburg O, Koopman M, Snippert HJG, Roodhart JML.
Impact of treatment history on drug resistance of metastatic colorectal cancer organoids.
PUBLICATIONS
iScience 2025
Nat Commun. 2025
Buissant des Amorie JR, Betjes MA, Bernink JH, Hageman JH, Geurts VE, Begthel H, Laskaris D, Heinz MC, Jordens I, Vinck T, Houtekamer RM, Verlaan-Klink I, Brunner SR, van Rheenen J, Gloerich M, Clevers H, Tans SJ, van Zon JS, Snippert HJG.
Intestinal tuft cell subtypes represent successive stages of maturation driven by crypt-villus signaling gradients.
Nat Genet. 2024
Kayhanian H*, Cross W*, van der Horst SEM*, Barmpoutis P*, Lakatos E*, Caravagna G, Zapata L, Van Hoeck A, Middelkamp S, Litchfield K, Steele C, Waddingham W, Patel D, Milite S, Jin C, Baker AM, Alexander DC, Khan K, Hochhauser D, Novelli M, Werner B, van Boxtel R, Hageman JH, Buissant des Amorie JR, Linares J, Ligtenberg MJL, Nagtegaal ID, Laclé MM, Moons LMG, Brosens LAA, Pillay N, Sottoriva A, Graham TA, Rodriguez-Justo M, Shiu KK, Snippert HJG#, Jansen M#.
Homopolymer switches mediate adaptive mutability in mismatch repair-deficient colorectal cancer.
Science 2024
Boon NJ, Oliveira RA, Körner PR, Kochavi A, Mertens S, Malka Y, Voogd R, van der Horst SEM, Huismans MA, Smabers LP, Draper JM, Wessels LFA, Haahr P, Roodhart JML, Schumacher TNM, Snippert HJ, Agami R, Brummelkamp TR.
DNA damage induces p53-independent apoptosis through ribosome stalling.
Cell reports 2023
Mertens S, Huismans MA, Verissimo CS, Ponsioen B, Overmeer R, Proost N, van Tellingen O, van de Ven M, Begthel H, Boj SF, Clevers H, Roodhart JML, Bos JL, Snippert HJG.
Drug-repurposing screen on patient-derived organoids identifies therapy-induced vulnerability in KRAS-mutant colon cancer.
Nature 2022
Azkanaz M, Corominas-Murtra B, Ellenbroek SIJ, Bruens L, Webb AT, Laskaris D, Oost KC, Lafirenze SJA, Annusver K, Messal HA, Iqbal S, Flanagan DJ, Huels DJ, Rojas-Rodríguez F, Vizoso M, Kasper M, Sansom OJ, Snippert HJ, Liberali P, Simons BD, Katajisto P, Hannezo E, van Rheenen J.
Retrograde movements determine effective stem cell numbers in the intestine.
Cancer Research 2022
Heinz MC*, Peters NA*, Oost KC, Lindeboom RGH, van Voorthuijsen L, Fumagalli A, van der Net MC, de Meideros G, Hageman JH, Verlaan-Klink I, Borel Rinkes IHM, Liberali P, Gloerich M, van Rheenen J, Vermeulen M, Kranenburg O#, Snippert HJG#.
Liver Colonization by Colorectal Cancer Metastases Requires YAP-Controlled Plasticity at the Micrometastatic Stage.
*Equal contribution
#Corresponding author
PLOS BIOLOGY 2022
Bollen Y*, Hageman JH*, van Leenen P, Derks LLM, Ponsioen B, Buissant des Amorie JR, Verlaan-Klink I, van den Bos M, Terstappen LWMM, van Boxtel R, Snippert HJG#.
Efficient and error-free fluorescent gene tagging in human organoids without double-strand DNA cleavage.
*Equal contribution
#Corresponding author
Nat Genet. 2021
Bollen Y*, Stelloo E*, van Leenen P, van den Bos M, Ponsioen B, Lu B, van Roosmalen MJ, Bolhaqueiro ACF, Kimberley C, Mossner M, Cross WCH, Besselink NJM, van der Roest B, Boymans S, Oost KC, de Vries SG, Rehmann H, Cuppen E, Lens SMA, Kops GJPL, Kloosterman WP, Terstappen LWMM, Barnes CP, Sottoriva A, Graham TA, Snippert HJG#.
Reconstructing single-cell karyotype alterations in colorectal cancer identifies punctuated and gradual diversification patterns.
*Equal contribution
#Corresponding author
Nat Commun. 2021
van Dijk E, van den Bosch T, Lenos KJ, El Makrini K, Nijman LE, van Essen HFB, Lansu N, Boekhout M, Hageman JH, Fitzgerald RC, Punt CJA, Tuynman JB, Snippert HJG, Kops GJPL, Medema JP, Ylstra B, Vermeulen L, Miedema DM.
Chromosomal copy number heterogeneity predicts survival rates across cancers.
Nat Cell Biol. 2021
Ponsioen B, Post JB, Buissant des Amorie JR, Laskaris D, van Ineveld RL, Kersten S, Bertotti A, Sassi F, Sipieter F, Cappe B, Mertens S, Verlaan-Klink I, Boj SF, Vries RGJ, Rehmann H, Vandenabeele P, Riquet FB, Trusolino L, Bos JL, Snippert HJG#.
Quantifying single-cell ERK dynamics in colorectal cancer organoids reveals EGFR as an amplifier of oncogenic MAPK pathway signalling.
#Corresponding author
STAR Protoc. 2020
Heinz MC, Oost KC, Snippert HJG#.
Introducing the Stem Cell ASCL2 Reporter STAR into Intestinal Organoids.
#Corresponding author
Developmental Cell 2020
Hageman JH, Heinz MC, Kretzschmar K, van der Vaart J, Clevers H, Snippert HJG#.
Intestinal Regeneration: Regulation by the Microenvironment.
#Corresponding author
Cell reports 2020
Bruens L, Ellenbroek SIJ, Suijkerbuijk SJE, Azkanaz M, Hale AJ, Toonen P, Flanagan DJ, Sansom OJ, Snippert HJ#, van Rheenen J#.
Calorie Restriction Increases the Number of Competing Stem Cells and Decreases Mutation Retention in the Intestine.
#Corresponding author
Cell reports 2020
de Witte CJ, Espejo Valle-Inclan J, Hami N, Lõhmussaar K, Kopper O, Vreuls CPH, Jonges GN, van Diest P, Nguyen L, Clevers H, Kloosterman WP, Cuppen E, Snippert HJG, Zweemer RP, Witteveen PO, Stelloo E.
Patient-Derived Ovarian Cancer Organoids Mimic Clinical Response and Exhibit Heterogeneous Inter- and Intrapatient Drug Responses.
Cell 2020
Beumer J*, Puschhof J*, Bauzá-Martinez J*, Martínez-Silgado A, Elmentaite R, James KR, Ross A, Hendriks D, Artegiani B, Busslinger GA, Ponsioen B, Andersson-Rolf A, Saftien A, Boot C, Kretzschmar K, Geurts MH, Bar-Ephraim YE, Pleguezuelos-Manzano C, Post Y, Begthel H, van der Linden F, Lopez-Iglesias C, van de Wetering WJ, van der Linden R, Peters PJ, Heck AJR, Goedhart J, Snippert H, Zilbauer M, Teichmann SA, Wu W, Clevers H.
High-Resolution mRNA and Secretome Atlas of Human Enteroendocrine Cells.
*Equal contribution
Cell Stem Cell 2020
Fumagalli A*, Oost KC*, Kester L, Morgner J, Bornes L, Bruens L, Spaargaren L, Azkanaz M, Schelfhorst T, Beerling E, Heinz MC, Postrach D, Seinstra D, Sieuwerts AM, Martens JWM, van der Elst S, van Baalen M, Bhowmick D, Vrisekoop N, Ellenbroek SIJ, Suijkerbuijk SJE, Snippert HJ, van Rheenen J.
Plasticity of Lgr5-Negative Cancer Cells Drives Metastasis in Colorectal Cancer.
*Equal contribution
Trends in Cancer 2020
Post JB, Roodhart JML, Snippert HJG#.
Colorectal Cancer Modeling with Organoids: Discriminating between Oncogenic RAS and BRAF Variants.
#Corresponding author
Cell 2020
Post Y*, Puschhof J*, Beumer J*, Kerkkamp HM, de Bakker MAG, Slagboom J, de Barbanson B, Wevers NR, Spijkers XM, Olivier T, Kazandjian TD, Ainsworth S, Iglesias CL, van de Wetering WJ, Heinz MC, van Ineveld RL, van Kleef RGDM, Begthel H, Korving J, Bar-Ephraim YE, Getreuer W, Rios AC, Westerink RHS, Snippert HJG, van Oudenaarden A, Peters PJ, Vonk FJ, Kool J, Richardson MK, Casewell NR, Clevers H.
Snake Venom Gland Organoids.
*Equal contribution
Mol Cancer Res 2020
Stangl C*, Post JB*, van Roosmalen MJ, Hami N, Verlaan-Klink I, Vos HR, van Es RM, Koudijs MJ, Voest EE#, Snippert HJG*, Kloosterman WP*#.
Diverse BRAF Gene Fusions Confer Resistance to EGFR-Targeted Therapy via Differential Modulation of BRAF Activity.
*Equal contribution
#Corresponding author
Nature Genetics 2019
Bolhaqueiro ACF*, Ponsioen B*, Bakker B, Klaasen SJ, Kucukkose E, van Jaarsveld RH, Vivié J, Verlaan-Klink I, Hami N, Spierings DCJ, Sasaki N, Dutta D, Boj SF, Vries RGJ, Lansdorp PM, van de Wetering M, van Oudenaarden A, Clevers H, Kranenburg O, Foijer F, Snippert HJG#, Kops GJPL#.
Ongoing chromosomal instability and karyotype evolution in human colorectal cancer organoids.
*Equal contribution
#Corresponding author
Nature Medicine 2019
Kopper O, de Witte CJ*, Lõhmussaar K*, Valle-Inclan JE*, Hami N, Kester L, Balgobind AV, Korving J, Proost N, Begthel H, van Wijk LM, Revilla SA, Theeuwsen R, van de Ven M, van Roosmalen MJ, Ponsioen B, Ho VWH, Neel BG, Bosse T, Gaarenstroom KN, Vrieling H, Vreeswijk MPG, van Diest PJ, Witteveen PO, Jonges T, Bos JL, van Oudenaarden A, Zweemer RP, Snippert HJG, Kloosterman WP, Clevers H.
An organoid platform for ovarian cancer captures intra- and interpatient heterogeneity.
Oncotarget 2019
Post JB, Hami N, Mertens AEE, Elfrink S, Bos JL, Snippert HJG#.
CRISPR-induced RASGAP deficiencies in colorectal cancer organoids reveal that only loss of NF1 promotes resistance to EGFR inhibition.
#Corresponding author
Nucleic Acids Res 2018
Bollen Y, Post J, Koo BK#, Snippert HJG#.
How to create state-of-the-art genetic model systems: strategies for optimal CRISPR-mediated genome editing.
#Corresponding author
Mol Syst Biol 2018
Lindeboom RG*, van Voorthuijsen L*, Oost KC, Rodríguez-Colman MJ, Luna-Velez MV, Furlan C, Baraille F, Jansen PW, Ribeiro A, Burgering BM, Snippert HJ#, Vermeulen M#.
Integrative multi-omics analysis of intestinal organoid differentiation.
*Equal contribution
#Corresponding author
Nat Commun 2018
Huels DJ, Bruens L, Hodder MC, Cammareri P, Campbell AD, Ridgway RA, Gay DM, Solar-Abboud M, Faller WJ, Nixon C, Zeiger LB, McLaughlin ME, Morrissey E, Winton DJ, Snippert HJ, van Rheenen J, Sansom OJ.
Wnt ligands influence tumour initiation by controlling the number of intestinal stem cells.
Cell Rep 2018
Oost KC, van Voorthuijsen L*, Fumagalli A*, Lindeboom RGH*, Sprangers J, Omerzu M, Rodriguez-Colman MJ, Heinz MC, Verlaan-Klink I, Maurice MM, Burgering BMT, van Rheenen J, Vermeulen M, Snippert HJG#.
Specific Labeling of Stem Cell Activity in Human Colorectal Organoids Using an ASCL2-Responsive Minigene.
#Corresponding author
Nat Protoc 2018
Fumagalli A, Suijkerbuijk SJE, Begthel H, Beerling E, Oost KC, Snippert HJ, van Rheenen J, Drost J.
A surgical orthotopic organoid transplantation approach in mice to visualize and study colorectal cancer progression.
Gastroenterology 2017
Bruens L, Ellenbroek SIJ, van Rheenen J#, Snippert HJ#.
In Vivo Imaging Reveals Existence of Crypt Fission and Fusion in Adult Mouse Intestine.
#Corresponding author
Curr Opin Syst Biol 2017
Ponsioen B, Snippert HJ#.
Cancer Systems Biology: Live imaging of intestinal tissue in health and disease.
#Corresponding author
Nature 2017
Rodríguez-Colman MJ, Schewe M, Meerlo M, Stigter E, Gerrits J, Pras-Raves M, Sacchetti A, Hornsveld M, Oost KC, Snippert HJ, Verhoeven-Duif N, Fodde R, Burgering BM.
Interplay between metabolic identities in the intestinal crypt supports stem cell function.
eLIFE 2016
Verissimo CS*, Overmeer RM*, Ponsioen B*, Drost J, Mertens S, Verlaan-Klink I, Gerwen BV, van der Ven M, van de Wetering M, Egan DA, Bernards R, Clevers H, Bos JL, Snippert HJ#.
Targeting mutant RAS in patient-derived colorectal cancer organoids by combinatorial drug screening.
*Equal contribution
#Corresponding author
Nature 2015
Drost J, van Jaarsveld RH*, Ponsioen B*, Zimberlin C*, van Boxtel R, Buijs A, Sachs N, Overmeer RM, Offerhaus GJ, Begthel H, Korving J, van de Wetering M, Schwank G, Logtenberg M, Cuppen E, Snippert HJ, Medema JP, Kops GJ, Clevers H.
Sequential cancer mutations in cultured human intestinal stem cells.
*Equal contribution
Nat Rev Cancer 2014
Vermeulen L# and Snippert HJ#.
Stem cell dynamics in homeostasis and cancer of the intestine.
#Corresponding author
Nature 2014
Ritsma L*, Ellenbroek SI*, Zomer A, Snippert HJ, de Sauvage FJ, Simons BD, Clevers H, van Rheenen J.
Intestinal crypt homeostasis revealed at single-stem-cell level by in vivo live imaging.
*Equal contribution
EMBO Rep 2014
Snippert HJ*#, Schepers AG*, Van Es JH, Simons BD, Clevers H.
Biased competition between Lgr5 intestinal stem cells driven by oncogenic mutation induced clonal expansion.
*Equal contribution
#Corresponding author
Science 2012
Schepers AG*, Snippert HJ*, Stange DE, van den Born M, van Es JH, van de Wetering M, Clevers H.
Lineage tracing reveals Lgr5+ stem cell activity in mouse intestinal adenomas.
*Equal contribution
Nat Protoc 2011
Snippert HJ, Schepers AG, Delconte G, Siersema PD, Clevers H.
Slide preparation for single-cell-resolution imaging of fluorescent proteins in their three-dimensional near-native environment.
EMBO Rep 2011
Snippert HJ and Clevers H.
Tracking adult stem cells.
Nature 2011
Sato T, Van Es JH, Snippert HJ, Stange D, Vries RG, Van den Born M, Barker N, Shroyer NF, Van de Wetering M, Clevers H.
Paneth cells constitute the niche for Lgr5 stem cells in intestinal crypts.
Cell 2010
Snippert HJ, Van der Flier LG, Sato T, Van Es JH, Van den Born M, Kroon-Veenboer C, Barker N, Klein AM, Van Rheenen J, Simons BD, Clevers H.
Intestinal crypt homeostasis results from neutral competition between symmetrically dividing Lgr5 stem cells.
Science 2010
Snippert HJ*, Haegebarth A*, Kasper M, Jaks V, van Es JH, Barker N, van de Wetering M, van den Born M, Begthel H, Vries RG, Stange DE, Toftgård R, Clevers H.
Lgr6 marks stem cells in the hair follicle that generate all cell lineages of the skin.
*Equal contribution
Cell Stem Cell 2010
Barker N*, Huch M*, Kujala P, van de Wetering M, Snippert HJ, van Es JH, Sato T, Stange DE, Begthel H, van den Born M, Danenberg E, van den Brink S, Korving J, Abo A, Peters PJ, Wright N, Poulsom R, Clevers H.
Lgr5(+ve) stem cells drive self-renewal in the stomach and build long-lived gastric units in vitro.
*Equal contribution
Nature 2009
Sato T, Vries RG, Snippert HJ, van de Wetering M, Barker N, Stange DE, van Es JH, Abo A, Kujala P, Peters PJ, Clevers H.
Single Lgr5 stem cells build crypt-villus structures in vitro without a mesenchymal niche.
Gastroenterology 2009
Snippert HJ, van Es JH, van den Born M, Begthel H, Stange DE, Barker N, Clevers H.
Prominin-1/CD133 marks stem cells and early progenitors in mouse small intestine.
Nat Genet 2008
Jaks V*, Barker N*, Kasper M*, van Es JH, Snippert HJ, Clevers H, Toftgård R.
Lgr5 marks cycling, yet long-lived, hair follicle stem cells.
*Equal contribution